Antibiotic resistance determination using Enterococcus faecium whole-genome sequences: a diagnostic accuracy study using genotypic and phenotypic data
Published in The Lancet Microbe, 2024
This work represents the largest evaluation to date on the accuracy of antibiotic resistance predictions from E. faecium genomes, using a dataset of 4,382 genomes with available culture-based AST phenotypes. I curated a catalogue of 228 genetic markers involved in resistance to 12 different antibiotics in E. faecium, and developed a bioinformatics pipeline to predict antibiotic resistance from E. faecium genomes, with important improvements in the accuracy of predictions. Given the mortality burden of antibiotic-resistant E. faecium and the increasingly routine use of WGS in clinical microbiology labs, these results and resources will facilitate the adoption of WGS as a tool for the diagnosis and surveillance of AMR in E. faecium.
Recommended citation: Coll F, Gouliouris T, Blane B, et al. Antibiotic resistance determination using Enterococcus faecium whole-genome sequences: a diagnostic accuracy study using genotypic and phenotypic data. January 11, 2024. The Lancet Microbe. DOI: 10.1016/S2666-5247(23)00297-5 https://papers.ssrn.com/sol3/papers.cfm?abstract_id=4345808